Abstract
Near-infrared absorption spectra of over 30 hydroxylated steroids dissolved in carbon disulfide were obtained with the Beckman DK-2 spectrophotometer. The use of microcuvettes made possible an analysis on less than 50 μg of steroid. The free hydroxyl group in the secondary and tertiary alcohols absorbed maximally at 2764–2772 mμ with extinction coefficients of 32–56 l/mole-cm, both parameters being affected by the position of the hydroxyl group. Hydroxyl groups attached to a carbon forming part of an epoxy linkage absorbed maximally at 2788–2798 mμ. α-Diols yielded doublets in specific locations, indicating intramolecular hydrogen bonding. 21-Hydroxy- and 17α-hydroxy-20-ketones gave broad bands with maximum absorption at 2867 and at 2843–2857 mμ, respectively, also ascribed to intramolecular hydrogen bonding. Suspensions of hydroxylated steroids in carbon disulfide also yielded characteristic spectra that differed from those of steroids in solution. The spectra of various non-steroid alcohols and diols dissolved in carbon disulfide were determined for comparison with those of hydroxylated steroids.
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