Near-Complete Genome Sequence of Clostridium paradoxum Strain JW-YL-7.

W Andrew Lancaster,Sagar M Utturkar,Steven D Brown,Dwayne A Elias,Dawn M Klingeman,Farris L Poole,Michael W W Adams

doi:10.1128/genomea.00229-16

Abstract

Clostridium paradoxum strain JW-YL-7 is a moderately thermophilic anaerobic alkaliphile isolated from the municipal sewage treatment plant in Athens, GA. We report the near-complete genome sequence of C. paradoxum strain JW-YL-7 obtained by using PacBio DNA sequencing and Pilon for sequence assembly refinement with Illumina data.

Highlights

Clostridium paradoxum strain JW-YL-7 is a moderately thermophilic anaerobic alkaliphile isolated from the municipal sewage treatment plant in Athens, GA
The anaerobic thermoalkaliphile Clostridium paradoxum strain JW-YL-7 was isolated from an aeration pool at the municipal sewage treatment plant in Athens, GA, USA, in 1993 [1], and representatives have been identified in sewage sludge from North America, Europe, and New Zealand [2].This organism grows optimally at 56°C at a pH of 10.1, with a doubling time of 16 min; produces acetate, H2, and CO2 as major products of fermentation; and has the unusual property of highly motile sporulated cells [1]
It has been proposed that C. paradoxum be assigned to the genus Peptoclostridium in the family Peptostreptococcacae, along with the pathogens Clostridium difficile and Clostridium sordellii and the alkalithermophilic Clostridium thermoalcaliphilum [3]

Summary

Introduction

Clostridium paradoxum strain JW-YL-7 is a moderately thermophilic anaerobic alkaliphile isolated from the municipal sewage treatment plant in Athens, GA. The anaerobic thermoalkaliphile Clostridium paradoxum strain JW-YL-7 was isolated from an aeration pool at the municipal sewage treatment plant in Athens, GA, USA, in 1993 [1], and representatives have been identified in sewage sludge from North America, Europe, and New Zealand [2].This organism grows optimally at 56°C at a pH of 10.1, with a doubling time of 16 min; produces acetate, H2, and CO2 as major products of fermentation; and has the unusual property of highly motile sporulated cells [1]. Previous studies have investigated the structure and function of the Naϩ translocating F1F0 ATPase of C. paradoxum, which functions strictly as an Naϩ exporter to establish an electrochemical gradient to drive cellular processes such as solute import and cell motility [4, 5].

Results

Conclusion