Abstract
Newcastle disease virus (NDV) is a high contagious disease, it infects respiration、digestion、nervous system and procreation, it is an an avian paramyxovirus。We propose a new adjuvant of mucosal─ polysaccharide viscosity liposome, inactive NDV encapsulated in liposomes and coated with Tremella or Xanthan Gum. In animal test，four-week-old specific-pathogen-free (SPF) white leghorn chickens, it was divided into five group of every group fetches 10 animals, after twice intranasal deliver, we collect its nasal wash liquid and serum to analyse the formulation amount of its specific sIgA and IgG with ELASA. The result verify, The titers of tremella-coated liposome-encapsulated NDV (T-lip) mucosal secretary immunoglobulin A (s-IgA) and serum IgG was the highest than the others（p＜0.05）. It display the highest ability of hemagglutination inhibition in HI TEST.（titer＝256）. After challenge with strong poisonous Sato of 1000MLD respectively, Chickens receiving virus alone had a 30% survival rate then NDV only、NDV-Liposome and Xanthan Gum coated liposome-encapsulated NDV had 60% survival rate. In contrast, groups that received T-lip had a higher survival rate of 100%. In immune system of polysaccharide viscosity liposome, T-lip phosphorylated in ERK pathway. To understand protection ability of polysaccharide viscosity liposome, we collected internal organs of alive chicken after challenge, example: throat、liver、lung and spleen, then we detected virus residue with RT-PCR. T-lip did not have virus residue in all internal organs almost. The result suggest that T-lip could enhance sIgA and IgG, then protected virus to invade in nasal mucosa immune system, it made survival rate up to 100%.
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