N-doped mesoporous carbon as a new sorbent for ultrasonic-assisted dispersive micro-solid-phase extraction of 1-naphthol and 2-naphthol, the biomarkers of exposure to naphthalene, from urine samples

Abstract

This study aimed to optimize a new sample preparation method using N-doped mesoporous carbon sorbent for simultaneous measurement of 1-naphthol and 2-naphthol, the biomarkers of exposure to naphthalene. The samples were analyzed using high-performance liquid chromatography supplied with ultraviolet detector (HPLC-UV). N-doped mesoporous carbon sorbent was obtained via the hard template procedure. The synthesized nanosorbent was then characterized by transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), and elemental analysis (CHN). The effective factors in the extraction of the studied biomarkers were examined by the Box-Behnken (BBD) methodology. Regarding the optimum conditions, the sketched calibration curve for naphthols was linear in the concentration levels of 1–600 µg L-1 for human urine samples. The accuracy and reproducibility of the introduced method were determined using the relative recovery (RR %) and relative standard deviation (RSD %) tests on the fortified urine samples. RR% and RSD% were found to be 97.0–101.2% and 3.1–9.0%, respectively. The calculated method detection limit of the optimized procedure was 0.3 µg L-1 and 0.5 µg L-1 for 1-naphthol and 2-naphthol, respectively.