Änderungen im Phosphat-Stoffwechsel regenerierender Zellen des Lebermooses Riella helicophylla (Bory et Mont.) Mont.

Abstract

In small thallus fragments with constant cell number (microfragments) of the liverwort Riella helicophylla acid soluble organic phosphate is doubled 48 h after fragment isolation. Nucleic acid phosphate increases 20–30 %, lipid-phosphate increases only slightly, inorganic phosphate diminishes approximately 50%. The additional phosphate is taken from the nutrient solution with growing tendency during the first 24 h. This supply, however, is dispensible, because the microfragments are able to regenerate in distilled water, indeed with delay and reduction. The registered shift of phosphate fractions is mainly caused by interrelationships between regenerating and non-regenerating cells within the fragment. This is revealed by microanalytical analysis of the adaxial and peripheral fragment region. The results show, that inorganic phosphate is enhanced in the regenerating cells, and diminished in the non-regenerating ones. The latter cells apparently serve as phosphate donators. 32 P is transferred quickly between the two cell types, in the isolated fragments in the same manner as in the intact plant. This leads to the conclusion that the phosphate augmentation in regenerating Riella cells is not brought about by new sink characteristics in these cells.