Abstract
Tomato spotted wilt virus (TSWV) is one of the most destructive plant viruses, causing severe losses in many important crops worldwide. The non-structural protein NSm of TSWV is a viral movement protein that induces viral symptoms. However, the molecular mechanisms by which NSm contributes to symptom development are unclear. Here, we present evidence that NSm directly interacts with Nicotiana benthamiana chloroplast thylakoid membrane protein TMP14 (NbTMP14) by yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays. The interaction between NSm and NbTMP14 led to the translocation of the NbTMP14 protein from the chloroplast to the cytoplasm in TSWV-infected plants, and overexpressing NSm decreased NbTMP14 mRNA accumulation. In addition, abnormal chloroplasts and starch accumulation were observed in TSWV-infected plants. Silencing of NbTMP14 by TRV VIGS also showed similar results to those of TSWV-infected plants. Overexpressing NbTMP14 in transgenic N. benthamiana plants impeded TSWV infection, and silencing NbTMP14 in N. benthamiana plants increased disease symptom severity and virus accumulation. To our knowledge, this is the first report showing that the plant chloroplast TMP14 protein is involved in viral infection. Knowledge of the interaction between NSm and NbTMP14 advances our understanding of the molecular mechanisms underlying TSWV symptom development and infection.
Highlights
Tomato spotted wilt virus (TSWV), a type member of the order Bunyavirales, family Tospoviridae and genus Orthotospovirus, is one of the most devastating plant viruses worldwide, causing severe economic losses to many important agronomic crops [1,2,3,4,5]
TSWV NSm Interacted with the N. benthamiana TMP14 Protein
The NSm protein is a pathogenic factor of the virus
Summary
Tomato spotted wilt virus (TSWV), a type member of the order Bunyavirales, family Tospoviridae and genus Orthotospovirus, is one of the most devastating plant viruses worldwide, causing severe economic losses to many important agronomic crops [1,2,3,4,5]. TSWV infects more than one thousand plant species from 84 different families, with typical symptoms including chlorosis, necrosis, ring spots, stunting, and ring/line patterns affecting leaves, stems, and fruits [6,7,8,9]. The M segment has ambisense polarity and encodes two glycoproteins (Gn and Gc) and a non-structural protein (NSm). The S segment is ambisense and encodes the nucleocapsid (N) protein and a second non-structural protein (NSs) [8,10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
