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Abstract
THE agglutination of homologous spermatozoa1,2 by heat-inactivated rabbit serum (56° C for 30 min) seems to be an immunological reaction. Although complement fixing activity of normal rabbit serum by rabbit spermatozoa was reported3,4, antibody-like activity was not proved. Edwards3 obtained neither immobilization of spermatozoa nor a positive reaction with passive cutaneous anaphylaxis and precipitin tests. Immunofluorescence6 and mixed conglutination7 gave similar results for spermagglutinin activity in normal rabbit serum. Germinal cells of guinea-pig testis have been lysed by the animal's own serum8, but contrary findings have been reported9–11 in which reaction between serum and homologous spermatozoa by immunofluorescence was negative. I have now investigated whether the spermagglutinin activity of normal rabbit serum is associated with antibody protein.
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