Abstract
SOIL-EXTRACT is a valuable constituent of media for the growth of many soil bacteria. A medium containing only agar and aqueous soil-extract will give much higher plate counts from soil than any other medium in common use. Lochhead and Chase1 showed that one-third of the bacterial cultures isolated from soil in soil-extract agar were unable to grow in a liquid medium containing a wide selection of amino-acids and vitamins, unless soil-extract was also added. They tentatively concluded that several different growth-promoting substances were involved. Some work on the nature of the soil-extract effect is briefly summarized here.
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