Abstract
1. 1. Digestion of experimentally induced connective tissue of man, dog and rat with trypsin solubilized about 30 per cent of the carbohydrate and peptide portions; and final digestion was accomplished with Pronase. 2. 2. Carbohydrate units yielded by this procedure, with only a few amino acid residues attached, were isolated by gel filtration and ion-exchange chromatography. 3. 3. Characterization of glycopeptides separated by these procedures indicated the presence of three distinct carbohydrate units: glucosylgalactosylhydroxylysine; plasma-type heteropolysaccharides consisting of galactose, mannose, glucosamine, fucose and sialic acid; and a mixture of glycosaminoglycans composed predominately of dermatan sulfate, chrondroitin-4 and/or -6 sulfates and hyaluronic acid.
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