Abstract

Experimental evidence has been presented that the non-ionic, acid-labile and base-stable bonds involving pectic carboxylate groups, both oriented and not-oriented, play an important role in holding pectic substances in the cell wall of Nitella. Participation of hydroxyl groups in the bonds has been confirmed by the vapor phase deuteration method. Irreversible acid-degradation of the cell wall ultrastructure associated with the carboxylate groups has also been demonstrated by acid-base titrimetry.

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