Abstract
IN 1966 Worthington1 described a method for the collection of hypophysial portal blood from the cut pituitary stalk of rats. This method was used by Fink, Nallar and Worthington2,3 to demonstrate the presence of luteinizing hormone releasing factor (LRF) in portal blood obtained from rats in proestrus and from rats hypophysectomized at least two weeks before collection. The chief disadvantage of this method of collection of portal blood was that contamination of samples by blood flowing back through pituitary sinusoids and bearing significant quantities of anterior pituitary hormones could not be prevented. Thus, because the ovarian ascorbic acid depletion method4,5 was used to assay the plasma, some of the activity exhibited by portal plasma from animals in proestrus probably resulted from luteinizing hormone (LH).
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