Abstract

HOS test is one of the widely used sperm function tests that was originally developed to assess membrane integrity of human spermatozoa. Recently, the test has been extended to assess sperm viability. Reliability of the HOS test in viability determination is conflicting. The purpose of the present study was to explore the distribution of the non hypo-osmotic tail swellings (NOS) in sperm samples, and to investigate their influence on the HOS test when used to assess sperm viability.

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