Abstract
CYTOPLASMICALLY inherited respiratory deficient mutants termed petites, were first described in baker's yeast over 20 years ago1. Since then in laboratory studies several species of yeast have been shown to give rise to respiratory deficient mutants2,3. However, although some respiratory deficient (obligatory fermentative) yeasts have been found in nature3, it is not clear whether these species are the result of cytoplasmic (petite) or chromosomal lesions4 because no suitable genetic tests are available. Nevertheless it is known that petite mutants of both Saccharomyces cerevisiae5 and Tornlopsis glabrata6 have large deletions from their mitochrondrial DNA with each independent isolate having a unique circular DNA size profile which can be regarded as a ‘fingerprint’. By contrast chromosomal or segregational mutants have intact and functional mitochondrial DNA, as shown by genetic tests3,7,8. Therefore it seemed possible that the type of lesion in naturally occurring respiratory deficient species could be determined by circular DNA profile analysis. Using this method, we show here that three independently isolated strains of Candida slooffii resemble petite mutants.
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