Abstract
Natural product chemistry laboratory in this research carried out a project-based laboratory related to the isolation of secondary metabolites from medicinal plants. The students learn the essential skills required to perform the extraction, fractionation, purification, and structural elucidation of secondary metabolites from medicinal plant. This study was conducted to isolate phenobarbitone from the bark of A. marina. The modified method uses maceration and recrystallization. This study obtained phenobarbitone 0.102 g from 100 g dried powder of bark of A. marina. Identified of phenobarbitone based on chromatogram of TLC, FT-IR, and UV-Vis.
Highlights
Natural product chemistry is one of the compulsory courses taken in third years by chemistry education students (Hakim & Jufri, 2018)
Natural product chemistry laboratory in this research carried out a project-based laboratory related to the isolation of secondary metabolites from medicinal plants
Identified of phenobarbitone based on chromatogram of Thin Layer Chromatography (TLC), FT-IR, and UV-Vis
Summary
Natural product chemistry is one of the compulsory courses taken in third years by chemistry education students (Hakim & Jufri, 2018). Natural product chemistry courses studied secondary metabolites, classification, structure, biosynthesis, and methods of isolation of terpenoid, steroids, flavonoids, polyphenols, alkaloids (Hakim & Jufri, 2017). One type of plant that can be used as a sample to be isolated in natural product chemistry is mangrove (Avicennia marina). Plants that can live in extreme areas like this, certainly have compounds that protect it from damage This is the reason that A. marina is very good as a sample in this study. According to Kordi (2012), mangrove plants contain secondary metabolite compounds, such as alkaloids, flavonoids, phenols, terpenoids, steroids and saponins. Based on the description above, it can be said that the phenobarbitone compound contained in the bark of mangrove has a property that can be utilized by the community
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