Abstract
Conventional flocculants bear environmental and health concerns which could be avoided by applying natural materials, particularly polysaccharide and glycoprotein-containing ones. In the present study, yeast cell wall (YCW), a natural polymer matrix, was used as natural flocculant. To prepare YCW, Saccharomyces cerevisiae was cultivated in bench scale fermenter. After characterization, YCW was employed as anionic flocculant in jar tests to remove turbidity from kaolin suspensions at different conditions where either alum or poly aluminum chloride (PAC) was coagulant. Generally, the lower coagulant consumption, higher turbidity removal or faster sedimentation was observed by using YCW as flocculant. The developed flocculant was more effective in the presence of PAC compared to alum. At best, by applying 300 mg/L YCW, the highest turbidity removals of 98 and 97% were achieved using 10 ppm PAC at pH 6.5 and 50 ppm alum at pH 7.5, respectively. The presence of the flocculant in the structure of the flocs was proved by FTIR analysis. The final pH of the treated suspensions was suitable for discharge purpose without the need for neutralization. The excess positive charge neutralization and bridging were the governing mechanism in coagulation-flocculation process. YCW with proper performance, GRAS designation and readily availability can be considered as natural alternative to chemical anionic flocculants where the process needs safe compounds.
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