Abstract
A survey across different agro-climatic regions of India was done and 38 groundnut seed samples were collected from various sources. Upon analysis, all samples were found infected with Aspergillus flavus ranging from 2 to 50% incidence with aflatoxin content of 0.0 to 28 ppb. Greenhouse studies revealed no correlation between incidence of A. flavus and aflatoxin content on seedling emergence, root length, shoot length and dry weight. Seeds were predominantly contaminated with aflatoxin B1 followed by aflatoxin B2. Among the tested A. flavus isolates, 31 were found aflatoxigenic and seven were nonaflatoxigenic when analyzed through cultural, thin layer chromatography, competitive direct enzyme linked immunosorbent assay and multiplex polymerase chain reaction. Present study reveals the current scenario of aflatoxin contamination, and aflatoxigenic and non-aflatoxigenic fungal infection in groundnut seeds collected across India. Keywords: Polymerase chain reaction (PCR), Aspergillus flavus , aflatoxin, enzyme-linked immuno sorbent assay (ELISA), groundnut African Journal of Biotechnology Vol. 12(19), pp. 2587-2597
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