Natural killer cell distribution and trafficking in normal and neoplastic human tissues

Abstract

Event Abstract Back to Event Natural killer cell distribution and trafficking in normal and neoplastic human tissues Paolo Carrega1*, Irene Bonaccorsi2, Emma Di Carlo3, Maria Cristina Mingari4, Lorenzo Moretta1 and Guido Ferlazzo2 1 Istituto Giannina Gaslini, Italy 2 University of Messina, Italy 3 University of Chieti-Pescara, Italy 4 University of Genoa, Italy Although natural killer (NK) cells have been detected in various lymphoid and non-lymphoid organs in mice, limited data are available regarding the distribution and trafficking of NK cells among human solid organs. To increase the current knowledge regarding the presence of NK cells in both healthy and pathological conditions, we analyzed a wide array of normal as well as neoplastic tissues derived from different human compartments. We found that NK cells were broadly distributed in most solid tissues, although, in general, the amount of NK cells significantly varied depending on the tissue/organ analyzed. Interestingly, NK cell distribution appeared to be subset-specific since some tissues were preferentially populated by CD56brightperforinlow NK cells while, others by the CD56dimperforinhigh cytotoxic counterpart. Accordingly, the gene expression level of chemotactic factors in the tissues analyzed matched the expression of chemokine receptors in the NK cell subsets infiltrating the tissues. Remarkably, the chemokine expression pattern of many human tissues resulted modified after neoplastic transformation. As a result, the relative proportion of the NK cell subsets infiltrating the tissues was different, in most cases toward a tumor- infiltrating NK cell population enriched in CD56brightperforinlow cells. Besides solid tissues, a CD3neg CD56bright NKp46+ cell population was also detected in seroma fluids, which represents an accrual of human afferent lymph, suggesting that NK cells might leave peripheral solid tissues. Overall, our results support the notion that distinct subsets of NK cells populate, and recirculate through, the majority of human peripheral tissues driven by organ-specific chemokine expression patterns. Keywords: NK cells, Human Tissue, Chemokines, trafficking, tumours Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Carrega P, Bonaccorsi I, Di Carlo E, Mingari M, Moretta L and Ferlazzo G (2013). Natural killer cell distribution and trafficking in normal and neoplastic human tissues. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01015 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Paolo Carrega, Istituto Giannina Gaslini, Genoa, Italy, pcarrega@unime.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Paolo Carrega Irene Bonaccorsi Emma Di Carlo Maria Cristina Mingari Lorenzo Moretta Guido Ferlazzo Google Paolo Carrega Irene Bonaccorsi Emma Di Carlo Maria Cristina Mingari Lorenzo Moretta Guido Ferlazzo Google Scholar Paolo Carrega Irene Bonaccorsi Emma Di Carlo Maria Cristina Mingari Lorenzo Moretta Guido Ferlazzo PubMed Paolo Carrega Irene Bonaccorsi Emma Di Carlo Maria Cristina Mingari Lorenzo Moretta Guido Ferlazzo Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.