Natural Extract of Moringa oleifera Leaves Promoting Control of Staphylococcus aureus strains biofilm on PVC surface

Abstract

Food-related diseases are a major challenge for public health and economic development, in which Staphylococcus aureus is often associated. The present study aimed to evaluate the effect of Moringa oleifera (MO) leaves against the biofilm and polysaccharide slime produced by two bacterial strains, S. aureus ATCC 25923 and S. aureus isolated from the dairy industry environment, on PVC surface. Inhibition of biofilm formation was observed within 15 min of treatment. Also, mature biofilms were significantly inhibited within less than 1 h of treatment. After 24 h of treatment, 99% of biofilm formation was inhibited for both S. aureus strains, with a reduction of 7 log CFU/cm2. As expected, 70% and 40% of polysaccharide slime formed by both S. aureus ATCC 25923 and the environmental strain reduced within 15 min and 30 min of treatment, respectively, reaching 99% reduction after 24 h of treatment with MO. Scanning electron microscopy images revealed the anti-biofilm potential effect of MO extract on the regular and irregular regions of the PVC surface. MO has proven to be effective in the control of biofilm formation by S. aureus on PVC surfaces; thus, it may be used as a sanitizer candidate in industrial surfaces.