Natural developing process of the cancer cachexia-inducing C26 colon carcinoma in mice

Abstract

Objective To observe the natural developing process of the cancer cachexia-inducing C26 colon carcinoma in mice.Methods The murine colon 26 adenocarcinoma cells was inoculated subcutaneously into BAL B/C mice.On the day O,5,10,15,20,and 25 after inoculation,body weight and gastroenemius muscle with epididymal adipose were documented. Biochemical parameters,serum tumor necrosis factor-α (TNF-α),the expression of nuclear factor-KB (NF-kB) in tumor and expression of ubiquitin proteasome E3 ligase ( Atrogin-1 ) and muscle ring finger protein 1 ( MURF-1 ) genese were valuated.Results Remarkable malnutrition and metabolic disorder occured about 15 days after the tumor inoculation in this model.There was significant difference in the concentrations of serum albumin between tumor-bearing group and healthy control group at the 15th day [(14.44 ±1.12) vs ( 19.80 ±2.45) g/L,P ＜0.01].The other biochemical indicators,TNF-α,NF-kB,and Atrogin-1 and MuRF-1 had beneficial changes to varying degrees ( P＜0.05 ).Conclusion Cancer cachexia development from the inoculated tumour cells to the obvious symptoms is a long process.The serum albumin may serve as an indicator to judge cancer cachexia development. Key words: Colon carcinoma; Cachexia; Model,animal