Abstract
Autoxidation of lipids is related to flesh deterioration and discoloration, as well as unpleasant flavors produced during processing and storage. This becomes a major factor determining fresh and frozen seafood products price. The aims of this research were to investigate the effect of two novel dietary supplementation antioxidants Vinlife ® grape seed extract and Herbalox ® rosemary extract on lipid oxidation during post-mortem storage of zebrafish ( Brachydanio rerio ) and the relationships between dietary antioxidants with the enzymatic antioxidant activities. Herbalox ® supplemented diet showed no harmful effects on growth or survival of zebrafish. However, the diet could not inhibit Thiobarbituric Acid Reactive Substances (TBARS) formation in zebrafish during post-mortem storage at 4 °C. Dietary Herbalox ® and Vinlife ® supplementation also showed no deleterious effects on growth or survival of zebrafish. The Vinlife ® supplementation efficiently suppressed TBARS formation in the zebrafish but the Herbalox ® supplementation. The two novel antioxidants had significant effects on catalase and glutathione S-transferase but not on glutathione peroxidase. These findings suggested that the supplementation with Vinlife ® was more effective than with Herbalox ® in inhibiting the formation of lipid oxidation products in zebrafish. Hence, Vinlife ® supplementation, could be a safe alternative method to improve oxidative stability of fish lipid contents.
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