Abstract

Although nitrogen isotopes are viewed as important tools for understanding plant N acquisition and allocation, the current interpretation of natural 15N-abundances (δ15N values) is often impaired by substantial variability among individuals or between species. Such variability is likely to stem from the fact that 15N-abundance of assimilated N is not preserved during N metabolism and redistribution within the plant; that is, 14N/15N isotope effects associated with N metabolic reactions are certainly responsible for isotopic shifts between organic-N (amino acids) and absorbed inorganic N (nitrate). Therefore, to gain insights into the metabolic origin of 15N-abundance in plants, the present paper reviews enzymatic isotope effects and integrates them into a metabolic model at the leaf level. Using simple steady-state equations which satisfactorily predict the δ15N value of amino acids, it is shown that the sensitivity of δ15N values to both photorespiratory and N-input (reduction by nitrate reductase) rates is quite high. In other words, the variability in δ15N values observed in nature might originate from subtle changes in metabolic fluxes or environment-driven effects, such as stomatal closure that in turn changes v0, the Rubisco-catalysed oxygenation rate.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.