Abstract
Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.
Highlights
Nils Hellwig,‡a Oliver Peetz,‡a Zainab Ahdash,b Igor Tascon, c Paula J
Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into styrene maleic acid lipid particles (SMALPs) keeping it in its native lipid surrounding
For a lot of analytical methods,[3,4,5] it poses new challenges for mass spectrometric (MS) analysis of membrane proteins: in contrast to conventional nanodiscs, which utilize a scaffold protein with a defined mass, stoichiometry, and diameter, the styrene-maleic acid (SMA) polymer exhibits a significant mass distribution and the diameter of the SMALPs and the number of polymers surrounding the protein in the SMALPs are not well defined
Summary
Nils Hellwig,‡a Oliver Peetz,‡a Zainab Ahdash,b Igor Tascon, c Paula J.
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