Abstract
Using an improved purification and derivatization procedure, the endogenous gibberellin- O-glucosides in mature runner beans ( Phaseolus coccineus) were analysed by combined gas chromatography-mass spectrometry. In addition to the previously characterized GA 1-3- O-glucoside and GA 8-2- O-glucoside, from runner beans, the following GA- O-glucosides were identified as endogenously occurring compounds by comparison of their full scan mass spectra and Kovats retention indices with those of standards: GA 1-13- O-glucoside, 3- epiGA 1-3- O-glucoside, GA 5-13- O-glucoside and GA 29-2- O-glucoside. The first three are reported in higher plants for the first time. The physiological relevance of the detected GA- O-glucosides is discussed.
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