Abstract
BackgroundThe Swedish National Cervical Screening Registry collects and evaluates comprehensive, nationwide health data to optimise organised cervical cancer prevention. Since all cervical cancer specimens are saved in biobanks, population-based data from the specimens should be available for analysis and linkage with other health information.MethodsWe identified all cervical cancers diagnosed in Sweden during 2002–2011 (4254 confirmed cases) and requested the tissue blocks to retrieve human papillomavirus (HPV) genotype data using general primer PCR with Luminex genotyping and real-time PCR targeting the E6/E7 regions of HPV16/18.ResultsWe obtained blocks from 2932/4254 (69%) of cases. Valid HPV genotyping data was retrieved for 2850 cases (97%). The most common type was HPV16 (60%), followed by HPV18 (19%), HPV45 (7%), HPV31 (3%), HPV33 (2%), HPV52 (2%), HPV39 (1%), HPV70 (1%), HPV56 (1%), HPV35 (1%), HPV58 (1%) and HPV59 (1%). Ninety-six percent of all HPV-positive cases had a single infection. Eighty-nine cases were HPV-positive only when testing for the HPV16/18-E6/E7 region.ConclusionsWe present one of the largest series of HPV-genotyped cervical cancers to date. The systematic collection of cervical cancer HPV genotyping data by the screening registry will facilitate prevention and monitoring of HPV type-specific disease burden.
Highlights
Human papillomavirus (HPV) infection is the major risk factor for cervical cancer.[1]
Effective screening methods exist and several effective prophylactic vaccines are licensed, cervical cancer is still common among women globally.[1]
Valid human papillomavirus (HPV) genotyping results were obtained for 2850/2932 cases (97%)
Summary
Human papillomavirus (HPV) infection is the major risk factor for cervical cancer.[1] effective screening methods (cytology and HPV testing) exist and several effective prophylactic vaccines are licensed, cervical cancer is still common among women globally.[1] Research on how effective health services are in real life and methods to obtain an evidence basis for how they could be improved is a priority. METHODS: We identified all cervical cancers diagnosed in Sweden during 2002–2011 (4254 confirmed cases) and requested the tissue blocks to retrieve human papillomavirus (HPV) genotype data using general primer PCR with Luminex genotyping and realtime PCR targeting the E6/E7 regions of HPV16/18. The systematic collection of cervical cancer HPV genotyping data by the screening registry will facilitate prevention and monitoring of HPV type-specific disease burden
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