Nasal eosinophilia and IL-5 mRNA expression in seasonal allergic rhinitis induced by natural allergen exposure: Effect of topical corticosteroids

Abstract

Background: Nasal allergen provocation in patients with allergic rhinitis leads to expression of the proeosinophilic cytokines IL-5 and GM-CSF and tissue eosinophilia. Objective: We sought to examine the effect of natural seasonal allergen exposure on IL-5 and GM-CSF mRNA expression and nasal eosinophilia and to evaluate the effects of topical corticosteroid therapy on these responses. Methods: Nasal biopsy specimens were collected from 46 grass pollen–sensitive patients with seasonal rhinitis before the grass pollen season. A second biopsy specimen was collected during the pollen season, by which time patients had received 6 weeks treatment with either fluticasone propionate (200 μg twice daily) or placebo nasal spray. Results: Fluticasone treatment was clinically effective ( P < .005). Patients receiving placebo, but not fluticasone, showed increased numbers of epithelial and submucosal EG2+ eosinophils ( P < .005) and IL-5 and GM-CSF mRNA–expressing cells ( P < .0001) during the pollen season. Colocalization experiments showed that greater than 80% of IL-5 mRNA–expressing cells were submucosal CD3+ T cells in both groups. The numbers of submucosal CD3+ T cells did not increase during the pollen season or decrease with fluticasone treatment. Fluticasone also inhibited IL-5 secretion by grass pollen–stimulated peripheral blood T cells from patients with seasonal rhinitis ( n = 5, inhibitory concentration of 50% = 10 –9 to 10 –10 mol/L). Conclusions: These results suggest that topical corticosteroids may reduce eosinophilia in seasonal rhinitis by inhibiting T cell IL-5 production. (J Allergy Clin Immunol 1998;102:610-7.)