Naphthalene-2-sulfonate induced toxicity in blood cells of freshwater fish Channa punctatus using comet assay, micronucleus assay and ATIR-FTIR approach

Abstract

Present inquisition was undertaken to evaluate the genotoxicity of naphthalene-2-sulfonate (2NS), a sulfonated aromatic compound and a momentous intermediate involved in the synthesis of dyes and surfactants, in fresh water fish, Channa punctatus. After LC50 determination, two sublethal concentrations i.e. 2.38 g/15 g b.w. (1/4 of LC50) and 4.77 g/15 g b.w. (1/2 of LC50) were selected for studying acute exposure. For evaluating sub chronic exposure 1/10th (0.238 g/L) and 1/20th (0.119 g/L) of safe application rate (SAR) were reckoned. Blood samples were collected after 24, 48, 72, and 96 h exposure period to study acute effect, and after 30 and 60 days exposure period for sub-chronic effect. Symbolic elevation in time and dose dependent DNA damage was observed by comet assay as well as micronucleus test revealing maximum damage after 60 days of exposure. After cessation of exposure to 2NS, evident recovery was observed after 30 days. Along with comet assay and micronucleus test, spectroscopic evaluation of DNA damage was also noted using Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR). The biomolecular range (800 cm−1 - 1800cm-1) in lyophilized red blood cell’s extracted from 60 days exposed as well as control group exhibit significant alterations in their nucleic acid indicated through multivariate analysis i.e. Principal Component Analysis (PCA). Further structural analysis of erythrocytes in maximally damaged group using Scanning Electron Microscopy was performed. Thus the study proposed the genotoxic impact of 2NS which is further supported by other toxicity markers like ATR-FTIR and Scanning Electron Microscopy.