Nanozyme Sensor Array Based on Porphyrin-Modified CoMoO4 to Detect and Distinguish Biologically Important Thiols

Abstract

In order to establish a rapid, accurate, and simple method for monitoring and analyzing the biological physiological state, we constructed a colorimetric sensor array based on the peroxidase-like activity of nanozymes (CoMoO4 and Por-CoMoO4) for the determination of five kinds of biothiols. The peroxidase-like activity of CoMoO4 has been greatly enhanced by the introduction of porphyrin (Por), due to the synergistic interaction between porphyrin and CoMoO4. Experimental data of the radical scavenger and fluorescence probe verify the generation of a superoxide radical (•O2–) during the catalytic reaction. Because different biothiols have different inhibiting effects on the catalytic activity of two nanozymes, each biothiol has a unique fingerprint with the aid of a chromogenic substrate (TMB). Through linear discriminant analysis, captopril (CAP), cysteine (Cys), homocysteine (Hcy), dithiothreitol (DTT), and glutathione (GSH) can be well identified in a certain range of concentrations. Different concentrations of biothiols and different proportions of mixed biothiols can be identified. It is expected that this study will provide a simple and rapid method for the identification of structural analogues, contributing to the monitoring of human life and health.