Abstract
Abstract Nanovid microscopy, nanometre-sized particles viewed with video-enhanced microscopy (1-3) is used to directly observe the lateral mobility of gold labelled membrane proteins and lipids, to observe the precise location and mobility of gold labelled pericellular and extracellular matrix macromolecules, and to follow endocytosis of gold labelled molecules. The technique involves the use of 30--40 nm colloidal gold particles conjugated to antibodies, streptavidin, lectins, or other ligands. The association of the antibody to the gold is non-covalent but extremely stable. Since a single colloidal gold particle is typically conjugated to many antibody molecules (4), an individual gold particle may bind to a small group of like membrane molecules rather than to a single molecule (5). The gold tag is visualized using video contrast enhancement to detect the light scattering of the gold particle. The gold can be viewed with bright-field, DIC, or epipolarization optics. Examples of bright-field and DIC images are shown in Figure 1.
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