Abstract
Photoluminescence (PL) quenching by gold nanoparticles (AuNPs) is a frequently applied principle in nanobiosensing. The quenching is most often explained in terms of the Forster resonance energy-transfer (FRET) mechanism, and more rarely in terms of the nanosurface energy-transfer (NSET) mechanism. Although both consider nonradiative resonance energy transfer, there are significant differences in predictions of the strength and the distance-dependence of the quenching. Here, we investigate the energy transfer to AuNPs from a terbium(III)-complex (Tb) with a long (millisecond) PL decay time with the aim to provide a better understanding of the underlying energy-transfer process. The binding of Tb-labeled streptavidin (Tb-sAv) to biotinylated AuNPs (biot-AuNPs) was studied using light-scattering spectroscopy. Quenching of the PL of Tb-sAv upon binding to biot-AuNPs of different diameters (5, 30, 50, 80 nm) was studied by time-resolved PL spectroscopy. Energy-transfer efficiencies were found to be practicall...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
