Nanosized hydroxyapatite and \u03b2-tricalcium phosphate composite: Physico-chemical, cytotoxicity, morphological properties and in vivo trial

Carlos Nelson Elias,Igor Da Silva Brum,Jorge José De Carvalho,Jorge Luis Da Silva Pires,Marco Antonio Alencar De Carvalho,Luiza Braga Ferreira Dos Santos

doi:10.1038/s41598-019-56124-4

Carlos Nelson Elias, Igor Da Silva Brum + Show 4 more

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https://doi.org/10.1038/s41598-019-56124-4

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Abstract

The objective of this work was to characterize the properties of a synthetic biomaterial composite with nanoparticles size (Blue Bone). This biomaterial is a composite recommended for dental and orthopedic grafting surgery, for guided bone regeneration, including maxillary sinus lift, fresh alveolus filling, and treatment of furcation lesions. The nano biomaterials surface area is from 30% to 50% higher than those with micro dimensions. Another advantage is that the alloplastic biomaterial has homogeneous properties due to the complete manufacturing control. The analyzed biomaterial composite was characterized by XRD, cytochemistry, scanning electron microscopy, porosimetry and in vivo experiments (animals). The results showed that the analyzed biomaterial composite has 78.76% hydroxyapatite [Ca5(PO4)3(OH)] with monoclinic structure, 21.03% β-tricalcium phosphate [β -Ca3(PO4)2] with trigonal structure and 0.19% of CaO with cubic structure, nanoparticles with homogeneous shapes, and nanoporosity. The in vivo experiments showed that the composite has null cytotoxicity, and the site of insertion biomaterials has a high level of vascularization and bone formation. The conclusion is that the synthetic biomaterial with Blue Bone designation presents characteristics suitable for use in grafting surgery applications.

Highlights

Igor da Silva Brum[1], Jorge José de Carvalho[1], Jorge Luis da Silva Pires[1], Marco Antonio Alencar de Carvalho[1], Luiza Braga Ferreira dos Santos 2 & Carlos Nelson Elias 2*
The analyzed biomaterial composite was characterized by X-ray diffraction (XRD), cytochemistry, scanning electron microscopy, porosimetry and in vivo experiments
The results showed that the analyzed biomaterial composite has 78.76% hydroxyapatite [Ca5(PO4)3(OH)] with monoclinic structure, 21.03% β-tricalcium phosphate [β -Ca3(PO4)2] with trigonal structure and 0.19% of CaO with cubic structure, nanoparticles with homogeneous shapes, and nanoporosity

Summary

Introduction

Igor da Silva Brum[1], Jorge José de Carvalho[1], Jorge Luis da Silva Pires[1], Marco Antonio Alencar de Carvalho[1], Luiza Braga Ferreira dos Santos 2 & Carlos Nelson Elias 2*. The objective of this work was to characterize the properties of a synthetic biomaterial composite with nanoparticles size (Blue Bone). This biomaterial is a composite recommended for dental and orthopedic grafting surgery, for guided bone regeneration, including maxillary sinus lift, fresh alveolus filling, and treatment of furcation lesions. Among the possible regions for collecting the patient’s bone, there are mandibular symphysis, calvaria, mandible, zygomatic bone, and ribs This kind of intervention may present high morbidity during the surgical procedure. In vivo studies analyzed differences in the numbers of osteocytes and osteoblasts in areas grafted with synthetic biomaterial composite of 70% hydroxyapatite and 30% calcium beta-phosphate, concluding that there was no statistical difference between groups when compared with native bone[2]. Nanohydroxyapatites should be comparable to natural bone grafts used for bone regeneration

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