Abstract
The monophoton counting technique was used to measure nanosecond time-resolved fluorescence emission spectra of 2-p-toluidino-naphthalene-6-sulfonate (2,6 p-TNS) adsorbed to lipid bilayer vesicles. A time-dependent red shift in the emission maximum was observed and the rate of this red shift was shown to be temperature dependent. Analysis of fluorescence decay curves obtained at different emission wavelengths indicates that the time-dependent spectral shifts are due to an excited-state reaction such as solvent relaxation or an excited-state interaction between the chromophore and a polar residue of the phospholipid.
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