Nanosecond Dynamics of Acetylcholinesterase Near the Active Center Gorge

Aileen E Boyd,Cristina S Dunlop,Lilly Wong,Zoran Radić,Palmer Taylor,David A Johnson

doi:10.1074/jbc.m401482200

Abstract

To delineate the role of peptide backbone flexibility and rapid molecular motion in acetylcholinesterase catalysis and inhibitor association, we investigated the decay of fluorescence anisotropy at three sites of fluorescein conjugation to cysteine-substitution mutants of the enzyme. One cysteine was placed in a loop at the peripheral site near the rim of the active center gorge (H287C); a second was in a helical region outside of the active center gorge (T249C); a third was at the tip of a small, flexible omega loop well separated from the gorge (A262C). Mutation and fluorophore conjugation did not appreciably alter catalytic or inhibitor binding parameters of the enzyme. The results show that each site examined was associated with a high degree of segmental motion; however, the A262C and H287C sites were significantly more flexible than the T249C site. Association of the active center inhibitor, tacrine, and the peripheral site peptide inhibitor, fasciculin, had no effect on the anisotropy decay of fluorophores at positions 249 and 262. Fasciculin, but not tacrine, on the other hand, dramatically altered the decay profile of the fluorophore at the 287 position, in a manner consistent with fasciculin reducing the segmental motion of the peptide chain in this local region. The results suggest that the motions of residues near the active center gorge and across from the Cys(69)-Cys(96) omega loop are uncoupled and that ligand binding at the active center or the peripheral site does not influence acetylcholinesterase conformational dynamics globally, but induces primarily domain localized decreases in flexibility proximal to the bound ligand.

Highlights

Catalysis of the hydrolysis of acetylcholine by the serine hydrolase, acetylcholinesterase (AChE),1 occurs at or near the diffusion limit [1, 2]
One cysteine was placed in a loop at the peripheral site near the rim of the active center gorge (H287C); a second was in a helical region outside of the active center gorge (T249C); a third was at the tip of a small, flexible ⍀ loop well separated from the gorge (A262C)
The results show that each site examined was associated with a high degree of segmental motion; the A262C and H287C sites were significantly more flexible than the T249C site

Summary

Nanosecond Dynamics of Acetylcholinesterase Near the Active Center Gorge*

The results suggest that the motions of residues near the active center gorge and across from the Cys69–Cys96 ⍀ loop are uncoupled and that ligand binding at the active center or the peripheral site does not influence acetylcholinesterase conformational dynamics globally, but induces primarily domain localized decreases in flexibility proximal to the bound ligand. Resolved fluorescence anisotropy yields information on the excited state of the fluorophore in the picosecond to nanosecond time frame [21], a time domain much shorter than the AChE catalytic cycle With this approach we hoped to assess the basic characteristics of the motion at sites near the gorge but across from the Cys69–Cys96 ⍀-loop. The very limited effects of ligand binding upon anisotropy decay suggest uncoupled movements of the regions examined, a finding consistent with a model for transient gorge openings that is dominated by random segmental movements

EXPERIMENTAL PROCEDURES

RESULTS

DISCUSSION

Normalized B factorb