Abstract
Scanning near-field optical/atomic-force microscopy (SNOAM) provided us with simultaneous topographic and fluorescence images of human chromosomes. The SNOAM uses a bent optical fiber simultaneously as a dynamic mode atomic force microscopy cantilever. Optical resolution was approximately 50—100 nm in fluorescence mode. Conventional karyotyping information was linked with SNOAM topographic analyses such as location of centromere and length of individual chromosomes. The height profile clearly indicated higher teromere regions. The SNOAM fluorescence images were different shapes from topographic images probably due to results from the combination of fluorescence dye and chromosome DNA.
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