Abstract
Dendritic cells (DCs) use finger-like filopodia, which are thin membrane protrusions that act as antennae, to probe the surrounding environment. Current strategies, such as cryo-EM, FIB-SEM, have revealed the filopodia structure in dendritic cells with nanoscale resolution; however, these techniques utilize fixed samples, limiting the information that can be gathered regarding the structural dynamics of these crucial structures in live cells. We utilized a fluorescent artificial antigen based on quantum dots (QDs) to visualize the filopodia structure in live murine DC2.4 cells.
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