Abstract
DNA-free genome editing using Cas9 ribonucleoprotein is advantageous because it introduces fewer potential undesirable genetic modifications than comparable methods. Here, we show a direct protein delivery system for plants using a cell-penetrating peptide-displayed polyion complex vesicle, CPP-PICsome, as a biological nanocarrier. With this system, we demonstrate nuclear genome editing by delivery of a Cas9 ribonucleoprotein complex in Arabidopsis thaliana callus.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
