Nanoscale Film Fabrication of Various Peptides on Neural Stem Cell Chip

Abstract

Modification of peptide on the electrode surface is very important issue for achieving valuable information from cell chip. In this study, various kinds of cysteine-containing peptide were fabricated on the electrode surface to enhance the electrochemical signals, cell spreading, and proliferation of rat neural stem cells. Different kinds of lysine-rich and RGD peptides were self-assembled on the gold nanoparticle modified ITO surfaces via strong Au-S chemical bond, followed by seeding neural stem cells (NE-4C) on its surface. As a result, K-MAP-C peptide consists of the quadruple branches of lysine chains and cysteine terminal showed outstanding characteristics respect to the improvement of redox signals, cell spreading and proliferation on electrode surface. Hence, our stem cell chip composed of lysine-rich peptide modified electrode can be usefully applied as efficient stem cell research tool.