Abstract
Pseudomonas aeruginosa Azurin is a metalloprotein of 14 kDa with copper ion in its active site. Protein film formation onto the substrate was performed by the self-assembly technique. In order to make molecule assembled layer onto the gold substrate, Azurin was cross-linked with N-succinimidyl-3-(2-pyridyldithio)-propionate (SPDP). SPDP have both a -thiol group to assembly with gold substrate and a linking part to assembly with protein. We detected optimized condition to make a protein film using Surface Plasmon Resonance (SPR). The surface structure of adsorbed Azurin film onto the gold surface was analyzed by scanning tunneling microscope (STM).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
