Abstract
Green technology for in vivo bioimaging is being developed for its biocompatibility and efficacy to serve as fluorescent probes in interior environment of the system. Herein, the preparation of nanoscale chlorophyll-liposome composite (NCLC) from the leaf extract of Tridax procumbens having near IR fluorescence is reported. The prepared NCLC has been characterized and confirmed using UV–vis spectroscopy, Fluorescence spectroscopy, Fourier transform infrared spectroscopy and Field emission-Scanning electron microscopic studies. Fluorescence microscopic images of Artemia nauplii and Penaeus vannamei after treatment with NCLC revealed its uptake evident from the appearance of red fluorescent granules in its alimentary canal which infers its possible utilization as an in vivo bioimaging tool (nanoprobes) on conjugation with specific antibodies for disease diagnosis in aquaculture.
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