Abstract
A nanoribbon biosensor (NRBS) was developed to register synthetic DNAs that simulate and are analogous to miRNA-17-3p associated with colorectal cancer. Using this nanoribbon biosensor, the ability to detect miRNA-17-3p in the blood plasma of a patient diagnosed with colorectal cancer has been demonstrated. The sensing element of the NRBS was a nanochip based on a silicon-on-insulator (SOI) nanostructure. The nanochip included an array of 10 nanoribbons and was designed with the implementation of top-down technology. For biospecific recognition of miRNA-17-3p, the nanochip was modified with DNA probes specific for miRNA-17-3p. The performance of the nanochip was preliminarily tested on model DNA oligonucleotides, which are synthetic analogues of miRNA-17-3p, and a detection limit of ~10−17 M was achieved. The results of this work can be used in the development of serological diagnostic systems for early detection of colorectal cancer.
Highlights
Colorectal cancer is a polyetiological disease and might be caused by a number of factors, including genetic and environmental factors
The study was aimed at determining the possibility of detecting miRNA-17-3p associated with colorectal cancer in biological samples using a nanoribbon biosensor
Legend: red curve represents pure 1 mM phosphate buffer (PPB), no target sDNA; blue curve shows target target sDNA with a concentration of C = 1.1 × 10−15−M, which is a synthetic analogue of miRNA-17-3p assDNA with a concentration of C = 1.1 × 10 15 M, which is a synthetic analogue of miRNA-17-3p sociated with colorectal cancer
Summary
Colorectal cancer is a polyetiological disease and might be caused by a number of factors, including genetic and environmental factors. Colorectal cancer is the second most common cancer-related mortality in the United States after lung cancer and is among the top three most common types of human cancer [1]. Since the early 2000s, there has been a significant decrease in the incidence of colorectal cancer due to both its early detection and effective therapy [1]. Among these disease diagnostics, colonoscopic screening [1] and fecal occult blood tests [2].
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