Abstract

Nowadays, achievement to easy, fast, reliable and cost-effective techniques and tools is one of the most important challenges in detection of microorganisms. Salmonella typhimurium is one of the most important pathogenic bacteria that affect the human health and environmental infections. In this work we report a new, stable, biocompatible and cost-effective platform for construction of an aptasensor based on nanoporous gold (NPG) for detection of S. typhimurium. Nanoporous gold is electrochemically synthesized using Au-Cu alloy at the surface of Au/GCE. Thiol functionalized aptamer is used for effective linking to the surface of NPG/Au/GCE via self-assemble monolayers (SAMs) formation. Morphological and voltammetric characterizations of the fabricated aptasensor were performed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The purposed aptasensor is capable to detect S. typhimurium in a wide linear dynamic range 6.5×102 to 6.5×108CFUmL−1 with a LOQ of 6.5×101CFUmL−1 and LOD of 1CFUmL−1. The prepared aptasensor showed some significant advantages such as sensitivity, selectivity, repeatability and ability to distinguish between live and dead bacteria cells. The prepared biosensor is successfully applied for salmonella detection in real samples such as egg.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.