Abstract
Using nanotechnology in the immunoassay field, Lynntech has developed a nanopore-based sensor with electrochemical signal transduction for the detection of biologically relevant molecular targets. Antibodies of interest were immobilized on the inside wall of nanopores and the antibody–antigen interaction was monitored by measuring the ionic conductance through the nanopores. We have used electrochemical impedance spectroscopy to monitor the changes in the ionic conductance due to the antibody–antigen interaction. To aid the development of a portable, fast immunoassay instrument, we have selected specific impedance frequency values that are very sensitive to the ionic conductance changes. Biomarkers of prostate cancer, prostate-specific antigen and hepsin, were successfully assayed by the nanopore membrane-based electrochemical immunoassay in both phosphate-buffered saline and plasma medium.
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