Abstract
AbstractConsidering the improved detection of biological analytes for affinity analysis is highly desirable, a metasurface plasmon resonance (Meta‐SPR)‐based imaging system, incorporating a localized SPR sensing platform with different microfluidic systems and employing simple bright‐field imaging, is established in this study. This system enables low‐level analyte concentration analysis, ranging from 100 pm to 100 nm, with the real‐time removal of nonspecific binding signals within the same device field of view. Combined with microfluidic systems and microdroplet spotting, it is possible to automatically measure the kinetic curves of a sample at ten concentration gradients or detect the specific responses of multiple samples in a single experiment simultaneously. This system can inexpensively and conveniently achieve complex detection functions, demonstrating an innovative breakthrough in sensor detection.
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