Abstract
C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods.
Highlights
The optical bioassay methods are the most widely used techniques in all the detection approaches
Gold nanoparticles (AuNPs)-coupled surface plasmon resonance (SPR) immunosensors have been explored to enhance the sensitivity of the detections system, and Au nanoparticles are recognized as SPR spectral sensitizers [1,6,7]
The microfluidic chip combined with total internal reflection imaging (TIRI) technology or SPR can be used as a point-of care testing (POCT) device for the detection of low concentration biomarkers [14,15,16,17,18]
Summary
The optical bioassay methods are the most widely used techniques in all the detection approaches. Researchers used an effective sandwich immune method to link Au nanoparticles on labeled antibodies, so as to achieve the binding complex on the surface of the sensor sensitive membrane, to achieve the effective amplification of the signal, so as to an obtain ultra-high resolution, which can amplify the detection signal several or even dozens of times. The gold and magnetic nanoparticles, which can increase the sensitivity of SPR, were used as labels on antibodies (Ab), forming composite particles in sandwich assays, and the increase in size and weight of composite particles helped to amplify SPR signals for the detection of biomarkers at very low concentrations. The microfluidic chip combined with total internal reflection imaging (TIRI) technology or SPR can be used as a point-of care testing (POCT) device for the detection of low concentration biomarkers [14,15,16,17,18]. The device could be used with the SPR, surface plasmon resonance imaging (SPRI), and TIRI during measurements
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