Abstract
AbstractThe Abbe resolution limit of ∼200 nm of conventional light‐optical microscopy greatly restricts the observation of subcellular processes. This Abbe resolution barrier has been surpassed by super‐resolution fluorescence microscopy methods that use time as an additional dispersing dimension. Light irradiation can be employed to externally control the emission properties of the luminescent markers, or spontaneous fluctuations of the emission of individual emitters can be analyzed. Here we review the development and application of light‐emitting nanoscale particles for super‐resolution imaging and discuss their (photo)physical and (photo)chemical characteristics to offer guidance in the selection of the optimal nanoparticle marker for an intended super‐resolution imaging experiment.
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