NANOPARTICLE BASED BIOSENSOR FOR PENICILLIN QUANTIFICATION IN PHARMACEUTICALS

Emine Karakus,Cisem Turan

doi:10.22159/ijap.2020v12i1.35906

Emine Karakus, Cisem Turan

Open Access

https://doi.org/10.22159/ijap.2020v12i1.35906

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Abstract

Objective: The objective of this study was to develop a new biosensor system based on nanoparticle to determine penicillin in pharmaceuticals. Methods: The characterization and optimization of the potentiometric penicillin biosensor (PB) were prepared by using synthesized surface-dependent and surface-independent ZnO nanoparticles named ZnO nanorods and chitosan were carried out. It was preferred ZnO nanorod because of its electrical, optical, physical and photocatalyst properties, biocompatibility and non-toxicity in the construction of the penicillin biosensor. Results: The operating range was obtained as 10-1-10-3M, the optimum buffer concentration was 10 mmol, optimum pH was 7.4 and the optimum temperature was 25 °C for the PB. The PB has advantages in terms of short response time, long enough shelf life, cheap, and easy elaborate. Conclusion: Whether the biosensor can be used to determine penicillin and accurately measure penicillin, the amount of penicillin in a commercial pharmaceutical preparation named Alfoxil was successfully made by using our prepared penicillin biosensor.

Highlights

Penicillin concentration is important in terms of biochemical and physiological reactions in the human body
Many potentiometric enzyme biosensors have been developed for the detection of penicillin G, mainly for the analysis of fermentation broths, where it requires the determination of relatively high concentrations of penicillin
Hexamethylenetetramine and zinc nitrate were used for the synthesis of nanorods, penicillinase (1150.39 Units/g) from Bacillus cereus, D-(+)-glucose monohydrate, chitosan, L-ascorbic acid, uric acid, zinc nitrate tetrahydrate sodium phosphate dibasic and monobasic sodium phosphate used in the study were purchased from Sigma Chem

Summary

Introduction

Penicillin concentration is important in terms of biochemical and physiological reactions in the human body. In order to determine antibiotics such as penicillin, there are different techniques such as microbiotic, chromotographic, spectrophotometric, fluorimetric and electrochemical. Electrochemical based penicillin biosensors are widely studied because the cost of preparation is low, the selectivity is high and the applicability is simple [1]. The determination of different kinds of penicillin are very important in medicine, pharmaceutical production, environmental monitoring and biochemical process control. G. drug control-analysis of antibiotic tablets, capsules and injectables, clinical laboratories, food control, etc.) are needed. Such sensors should possess both high stability in the long-term as well as a low detection limit [2]

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