Nanomaterized zinc sulfide-meerschaum biomatrix efficiently suppressed Fusarium verticilloides with augmented rice seed quality benefits during storage.

Abstract

The seed-borne mycopathogen Fusarium verticilliodes is a serious and deleterious pathogen causing substantial losses of rice seeds and grains. Rice seeds are prone to infestation at all points of the production chain and the fungal pathogen continues dormant devastation even during storage, adversely affecting the seed parameters. Its control is compromised due to the nonavailability of recommended fungicides during storage. Nanotechnological interventions can provide effective and ecofriendly alternative against mycopathogens during resting periods. Herein, the zinc sulfide-meerschaum nano bio-matrix (nZnS-MR) is presented to show this strategy, which worked well against F. verticilliodes when applied on freshly harvested rice seeds during 6 months of storage. The healthy, smooth and rounded girths of F. verticilloides mycelium were reduced with loss of turgidity, disrupting the hyphal exterior architecture, during in vitro treatment with nZnS-MR, endorsed by staining methodology, crystal violet and intracellular soluble protein leakage assays. In vivo application on rice seeds optimized 750 μg g-1 of nano zinc sulfide (nZnS) for 6 months of application during storage with maximum reduction of disease parameters [seedling blight (1.19%) and seed rot (5.43%)] and most augmented quality parameters [maximum germination (94.14%), seedling length (22.50 cm), dry weight (0.121 g) and vigor index (11.37)]. nZnS-MR acted as a slow release nanoformulation of nZnS for long-term antifungal activity. nZnS-MR is presented as an ecofriendly, biocompatible, bio-efficient, profertilization, cost-effective green material for the control of F. verticilliodes with rice seed invigorating effect, describing it as new a nano-generation material for efficient storage application.