Abstract
Electrochemical detection strategies employing nanotechnologies which include a variety of new materials and fabrication processes offer new opportunities for highly sensitive detection of cancer biomarkers. This work reports an electrochemical biosensor based on a graphene (GR) platform, combined with magnetic beads (MBs) and enzyme-labeled antibody-gold nanoparticle bioconjugates. MBs coated with capture antibodies (Ab1) were attached to isolated GR sheets by an external magnetic field, to avoid reducing the conductivity of graphene. Sensitivity was also enhanced by modifying the gold nanoparticles (AuNPs) with horseradish peroxidase (HRP) and the detection antibody (Ab2), to form the conjugate Ab2-AuNPs-HRP. Electron transport between the electrode and analyte target was accelerated by the multi-nanomaterial, and the limit of detection (LOD) for carcinoembryonic antigen (CEA) reached 5ng/mL, which meets clinical requirements. The multi-nanomaterial electrode GR/MBs-Ab1/Antigen/Ab2-AuNPs-HRP can be used to detect biomolecules such as CEA. The EC biosensor is sensitive and specific, and has potential in the detection of disease markers.Keywords: electrochemical, graphene,magnetic beads, gold nanoparticles, horseradish peroxidase.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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