Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules.

Minghua Lu,Xueqing Yang,Zongwei Cai,Yixin Yang,Xiuru Wu,Peige Qin

doi:10.3390/nano7040087

Minghua Lu, Xueqing Yang + Show 4 more

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https://doi.org/10.3390/nano7040087

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Journal: Nanomaterials	Publication Date: Apr 21, 2017
Citations: 82	License type: CC BY 4.0

Affiliation: Henan University, Hong Kong Baptist University

Abstract

Matrix-assisted laser desorption/ionization (MALDI), a soft ionization method, coupling with time-of-flight mass spectrometry (TOF MS) has become an indispensible tool for analyzing macromolecules, such as peptides, proteins, nucleic acids and polymers. However, the application of MALDI for the analysis of small molecules (<700 Da) has become the great challenge because of the interference from the conventional matrix in low mass region. To overcome this drawback, more attention has been paid to explore interference-free methods in the past decade. The technique of applying nanomaterials as matrix of laser desorption/ionization (LDI), also called nanomaterial-assisted laser desorption/ionization (nanomaterial-assisted LDI), has attracted considerable attention in the analysis of low-molecular weight compounds in TOF MS. This review mainly summarized the applications of different types of nanomaterials including carbon-based, metal-based and metal-organic frameworks as assisted matrices for LDI in the analysis of small biological molecules, environmental pollutants and other low-molecular weight compounds.

Highlights

Matrix-assisted laser desorption/ionization (MALDI), a powerful soft ionization method developed by Tanaka [1] and Karas [2], coupled with time-of-flight mass spectrometry (TOF MS)has become an indispensable analytical tool in complex sample analysis [3,4,5,6,7,8]
The results demonstrated that PS/OCNTs film was a good TFME adsorbent toward the analytes and an excellent thin film microextraction (TFME) and matrix of LDI-TOF MS for the analysis of BaP from water
We have reviewed the application of a series of nanomaterials, including carbon based materials, metal-based materials and metal organic frameworks, as matrices in LDI-TOF MS for the analysis of small molecules, biological molecules and environmental pollutants, with the publications from 2010

Summary

Introduction

Matrix-assisted laser desorption/ionization (MALDI), a powerful soft ionization method developed by Tanaka [1] and Karas [2], coupled with time-of-flight mass spectrometry (TOF MS). Considerable efforts have been made for developing various techniques and methods to solve the problems of MALDI-TOF MS in the analysis of low molecular weight compounds [11,12,13,14]. Due to the interference in low-molecular weight region of the traditional organic matrices, MALDI was seldom applied in analysis of small biological molecules and environmental pollutants in the past. Nanomaterials including carbon-based, metal-based and metal organic frameworks (MOF) based materials that were applied as the assisted matrices for LDI for the analysis of small biological molecules, environmental pollutants, and other low-molecular weight compounds, were reviewed, with a focus on publications from 2010 to present

Nanomaterial-Assisted LDI Method Development

Summary

Nanomaterial-Assisted LDI for the Analysis of Small Biological Molecules

Nanomaterial-Assisted LDI for the Analysis of Environmental Pollutants

Nanomaterial-Assisted LDI for the Analysis of Other Small Molecules

Findings

Conclusions