Abstract
Micrometer-resolution mass spectrometric imaging of live hippocampal tissue is achieved with a highly efficient desorption of biomolecules using a 532 nm continuous wave laser and gold nanoparticles or graphene oxide as an energy transporter, which enables clear identification of the distributions of monoacylglycerol, adenine, cholesterol, sphingosine and ceramide.
Highlights
Micrometer-resolution mass spectrometric imaging of live hippocampal tissue is achieved with a highly efficient desorption of biomolecules using a 532 nm continuous wave laser and gold nanoparticles or graphene oxide as an energy transporter, which enables clear identification of the distributions of monoacylglycerol, adenine, cholesterol, sphingosine and ceramide
The atmospheric pressure mass spectrometry (AP-MS) system for this study is described in detail in Electronic supplementary information (ESI) Note 1 and Fig. S1.† A 532 nm CW laser beam was introduced into the inverted microscopy by a dichromic beam splitter (NFD01-532-25x36, Semrock, USA) and was focused precisely on the specimen through the objective lens (Scheme 1)
We focused on cornu ammonis 1 (CA1), cornu ammonis 3 (CA3), and dentate gyrus (DG), since the connections among these three regions play an important role in consolidating information of short-term memories to long-term memories and spatial navigation.[24,25,26]
Summary
Micrometer-resolution mass spectrometric imaging of live hippocampal tissue is achieved with a highly efficient desorption of biomolecules using a 532 nm continuous wave laser and gold nanoparticles or graphene oxide as an energy transporter, which enables clear identification of the distributions of monoacylglycerol, adenine, cholesterol, sphingosine and ceramide. Scheme 1 Visible-light-absorbing nanomaterials and CW-laser-based mass spectrometry imaging system for live hippocampal tissue slice. (1) Incubating nanomaterials on tissue, (2) CW-based desorption and ionization with laser and plasma, (3) mass-based spectrometry imaging process.
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