Abstract
A sandwich-type electrochemical aptasensor was designed for sensitive detection of leptin in biological samples, including human serum and human plasma. The developed aptasensor was produced by electrodeposition of gold nanoparticles on a screen-printed electrode modified with zinc oxide nanoparticles. The synergy effect of zinc oxide and gold nanoparticles improved the electrocatalytic activity of the aptasensor. The obtained high surface area allowed more aptamer molecules to be loaded on the electrode surface. Signal amplification significantly increases the detection sensitivity of a developed biosensor. Although the use of nanomaterials is the most preferred detection tool for this purpose, as an alternative, enzyme-catalyzed signal amplification is widely used in the construction of a biosensor due to its specificity and high catalytic efficiency. Therefore, both nanomaterial-supported and an alkaline phosphatase-based aptasensor design were developed, which can produce in situ electroactive product by enzymatic hydrolysis of the inactive substrate to achieve a higher signal-to-background ratio. Under optimal conditions, the developed aptasensor exhibited a wide linear concentration range from 0.01 pg mL-1 to 100.0 pg mL-1 with a detection limit of 0.0035 pg mL-1. While the developed aptasensor provided excellent selectivity in the presence of some interfering compounds, it possessed outstanding reproducibility and stability. In addition, the developed aptasensor has been applied with good recoveries in the range 96.31 to 108.79% in human serum and plasma samples. In conclusion, all the obtained results showed the feasibility of the developed aptasensor for practical applications.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
